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While Illumina ultimately opted for a method that simultaneously detected massive numbers of DNA strands, others still believed that single-molecule sequencing methods offered a better path forward. Sequencing by synthesis requires repeated amplification, which introduces the possibility of error at each step and biases outputs towards sequences readily amplified by DNA polymerase. Single-molecule techniques were billed as a way to sequence DNA with minimal bias while simultaneously reducing cost.
</span></span><span style="display:flex"><span> - <span style="color:#ae81ff">./data:/etc/linkding/data</span>，这一点在下载安装汽水音乐中也有详细论述